Coding

Part:BBa_K5043010:Design

Designed by: Richard Meuten   Group: iGEM24_TUDarmstadt   (2024-09-12)


phtAd from M. vanbaalenii Pyr-1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 778
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 778
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 778
    Illegal XhoI site found at 1124
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 778
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 778
    Illegal NgoMIV site found at 1159
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For GoldenBraid-Assemble internal BsaI and BsmbI restriction sides were removed.


Source

Derived from Mycobacterium vanbaalenii Pyr-1's genome.

References